Recombinant human basic fibroblast growth factor rh-bFGF,rhbFGF,FGF

Description: bFGF is a multifunction nutritional cell factor, it is first discovered that bFGF has the mitosis activating effects with cultured fibroblast, later it is discovered that bFGF also has mitosis activating effects on many other cells, especially to mesoderm and neuroectoderm derived cells. Besides the mitosis activating effects, bFGF also has many other biological functions, such as inducing cell to change chemotaxis property, inducing or inhibiting the expression of certain cell type specific proteins, regulating cell differentiation, also involved in the functioning of endocrine system and neural system. Recombinant human FGF is composed with 155 amino acids, the human rh-bFGF coding cDNA was obtained from Peripheral blood leukocytes mRNA, its cDNA was synthesized by reverse-transcription, and it was subcloned into expression vector and expressed in E.coli DH5a.The recombinant human bFGF is purified by our unique biochemical purification method.

Purity:> 95%, as determined by SDS-PAGE analysis.

Molecular Weight: 17.0±10% kDa

Activity: the rh-bFGF is fully biologically active compared with certain standards. The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells (PNAS 94,10205,1997) is < 1 ng/mL, corresponding to a specific activity of > 1x10(E6) units/mg.
Formulation: Lyophilized from a 0.22mm filtered solution in 20 mM PB in absence of any carrier protein.

Storage: Lyophilized samples are stable for greater than two years at 2ºC to 8ºC. rh_bFGF in the solution should stored at -20ºC to -70ºC for six monthes.Avoid repeated freeze-thaw cycles.

Related:aFGF (GMP)