Recombinant human vascular endothelial cell growth factor-165(rh-VEGF165)
Description:VEGFs are a group of  polypeptide growth factors, and they are classified as a member of the platelet-derived growth factor family. They are very potent vascular permeability factors and specific mitogens for vascular endothelial cells, and possess strong angiogenesis in vivo. Among them, VEGF165 is the best potent chemoattractant molecule for monocytes and endothelial cells.
Recombinant Human Vascular Endothelial Growth Factor (rh-VEGF₁₆₅), a 20 kDa protein consisting of 165 amino acid residues, is produced as a homodimer protein secreted from yeast (Pichia pastori). It is apparently a homodimer according to reduced and non-reduced SDS-PAGE gels, but preparations of VEGF165 show some heterogeneity, depending on the constitution of different glycosylation patterns. All forms possess similar biological activities.
Source: Yeast (Pichia pastoris) Expressed
Molecular Weight: 42kDa
Specific Activity: 1.0 x 10⁵ U/mg
Purification: Affinity chromatography using Heparin-Sepharose FF as matrix.
Sterility: Filtered through a 0.22μm sterile filter.
Purity:>90%, by SDS-PAGE and visualized by silver stain
Endotoxin Level: <0.5EU /mg of rh-VEGF₁₆₅
Buffer: 20mM PB,pH7.4
Activity:ED50 = 1~6 ng/ml, determined by the dose dependent proliferation of human umbilical vein cells.Optimal concentration for individual application should be determined by a dose response assay.
Stability:Liquid rh-VEGF_165,although stable at room temperature for no less than 2 weeks,should be stored it below -18℃. For long term storage it is recommended to add a stabilizer PEG4000 (0.1%).Please avoid freeze-thaw cycles.