Recombinant Human GM-CSF,GMCSF,rHuGM-CSF
A DNA sequence encoding the mature human GM-CSF protein sequence
was expressed in E.coli.
Molecular Mass The 127 amino acid residue recombinant protein has a
molecular mass of approximately 14.5kD.
Purity > 97%, as determined by SDS-PAGE and
Endotoxin Level <10EU/0.3mg of the cytokine as
determined by the LAL method.
Activity Measured in a cell proliferation assay using the
factor-dependent cell line,TF-1, the specific activity shall be not less than 1 107IRU/mg.
Formulation Lyophilized from a 0.2µm filtered solution in PBS containing
human serum albumin per 1µg of cytokine.
Reconstitution It is recommended that sterile ddH2O containing at least
serum albumin or bovine serum albumin be added to the vial to prepare
a stock solution of no less than 1 µg/mL of the cytokine.
Storage Lyophilized samples are stable for greater than six months from
receipt at -20 to -70ºC .
Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8ºC for one month or at -20 to -70ºC in a manual defrost freezer for three months without detectable loss of activity.
Avoid repeated freeze-thaw cycles.
Size and prices 100?/font>g
GM-CSF was initially characterized as a growth factor that can support the in
vitro colony formation of granulocyte-macrophage progenitors. It is produced by
a number of different cell types (including activated T cells,B cells,macrophages,mast cells, endothelial cells and fibroblasts) in response to
cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage
progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and
eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival
factor for and activates the effector functions of granulocytes,
monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a
functional role on non-hematopoietic cells. It can induce human endothelial
cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the
proliferation of a number of tumor cell lines,including osteogenic sarcoma,
carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human
GM-CSF has no biological effects on mouse cells.