Manufacturer of Pdgf Metallothionein


Human Granulocyte-Macrophage Colony Stimulating Factor

Manufacturer,provider and supplier of
Recombinant Human GM-CSF,GMCSF,rHuGM-CSF

Source    A DNA sequence encoding the mature human GM-CSF protein sequence was expressed in E.coli.
Molecular Mass The 127 amino acid residue recombinant protein has a predicted molecular mass of approximately 14.5kD.
Purity      > 97%, as determined by SDS-PAGE and HPLC method.
Endotoxin Level    <10EU/0.3mg of the cytokine as determined by the LAL method.
Activity   Measured in a cell proliferation assay using the factor-dependent cell line,TF-1, the specific activity shall be not less than 1 107IRU/mg.
Formulation Lyophilized from a 0.2m filtered solution in PBS containing 50g of human serum albumin per 1g of cytokine.
Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 g/mL of the cytokine.
Storage  Lyophilized samples are stable for greater than six months from date of receipt at -20 to -70C .
Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8C for one month or at -20 to -70C in a manual defrost freezer for three months without detectable loss of activity.
Avoid repeated freeze-thaw cycles.
Size and prices 100?/font>g US$200,1.0mg US$1,100

GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells,B cells,macrophages,mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines,including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells.


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