Recombinant Human Interferon
Desription Interferon-a 2b Human Recombinant produced in E.Coli is a single,
non-glycosylated, polypeptide chain containing 166 amino acids and having a
molecular mass of 19400 Dalton.The Interferon-alpha 2b gene was obtained from
human leukocytes.The IFN-a 2b is purified by proprietary chromatographic
Source Escherichia Coli.
Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a (1 mg/ml) solution in containing 2.3 mg Sodium
phosphate dibasic and 0.55 mg sodium phosphate monobasic buffer.
Solubility It is recommended to reconstitute the lyophilized Interferon-alpha 2b
in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted
to other aqueous solutions.
Stability Lyophilized Interferon although stable at room temperature for 3
weeks, should be stored desiccated below -18°C. Upon reconstitution IFN alpha 2b
should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or
Please prevent freeze-thaw cycles.
Purity >98.0% as determined by RP-HPLC and SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was
determined and was found to be Met-Cys-Asp-Leu-Pro.
Activity The specific activity as determined in a viral resistance assay using
bovine kidney MDBK cells was found to be 260,000,000 IU/ mg.
Usage For LABORATORY RESEARCH USE ONLY. The product may not be used as drugs,
agricultural or pesticidal products, food additives or household chemicals.
1 Recombinant Human
Interferon alpha 2a (Reagent)
2 Recombinant Human
Interferon alpha 2b (Material)