Manufacturer of Pdgf-bb rh-VEGF Zn-MT

 
 

rh-VEGF

 

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Recombinant Human VEGF-165

Manufacturer,provider and supplier of
Recombinant human vascular endothelial cell growth factor-165(rh-VEGF165)


Description:
VEGFs are a group of  polypeptide growth factors, and they are classified as a member of the platelet-derived growth factor family. They are very potent vascular permeability factors and specific mitogens for vascular endothelial cells, and possess strong angiogenesis in vivo. Among them, VEGF165 is the best potent chemoattractant molecule for monocytes and endothelial cells.
Recombinant
Human Vascular Endothelial Growth Factor (rh-VEGF165), a 20 kDa protein consisting of 165 amino acid residues, is produced as a homodimer protein secreted from yeast (Pichia pastori). It is apparently a homodimer according to reduced and non-reduced SDS-PAGE gels, but preparations of VEGF165 show some heterogeneity, depending on the constitution of different glycosylation patterns. All forms possess similar biological activities.


Source:
Yeast (Pichia pastoris) Expressed

Molecular Weight:
42kDa

Specific Activity:
1.0 x 105 U/mg

Purification:
Affinity chromatography using Heparin-Sepharose FF as matrix.

Sterility:
Filtered through a 0.22
μm sterile filter.


Purity
:>
90%, by SDS-PAGE and visualized by silver stain

Endotoxin Level:
<0.5EU /mg of rh-VEGF165

Buffer:
20mM PB,
pH7.4


Activity
:ED50 = 1~6 ng/ml, determined by the dose dependent proliferation of human umbilical vein cells.
Optimal concentration for individual application should be determined by a dose response assay.

Stability:
Liquid rh-VEGF165,although stable at room temperature for no less than 2 weeks,should be stored it below -18
. For long term storage it is recommended to add a stabilizer PEG4000 (0.1%).Please avoid freeze-thaw cycles.


 

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