vascular endothelial cell growth factor-165(rh-VEGF165)
are a group of polypeptide growth factors, and they are classified as a member
of the platelet-derived growth factor family. They are very potent vascular
permeability factors and specific mitogens for vascular endothelial cells, and
possess strong angiogenesis in vivo. Among them, VEGF165 is the best
potent chemoattractant molecule for monocytes and endothelial cells.
Recombinant Human Vascular
Endothelial Growth Factor (rh-VEGF165), a 20 kDa protein consisting
of 165 amino acid residues, is produced as a homodimer protein secreted from
yeast (Pichia pastori). It is apparently a homodimer according to reduced
and non-reduced SDS-PAGE gels, but preparations of VEGF165 show some
heterogeneity, depending on the constitution of different glycosylation
patterns. All forms possess similar biological activities.
Yeast (Pichia pastoris) Expressed
Specific Activity: 1.0 x 105
chromatography using Heparin-Sepharose FF as matrix.
Sterility: Filtered through
by SDS-PAGE and visualized by silver stain
<0.5EU /mg of rh-VEGF165
Buffer: 20mM PB,pH7.4
Activity:ED50 = 1~6 ng/ml, determined by
the dose dependent proliferation of human umbilical vein cells.Optimal
concentration for individual application should be determined by a dose response
rh-VEGF165,although stable at room temperature for no less than 2
weeks,should be stored it below -18℃.
For long term storage it is recommended to add a stabilizer PEG4000 (0.1%).Please
avoid freeze-thaw cycles.